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M94A2684.TXT
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1994-10-25
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Document 2684
DOCN M94A2684
TI Purification, processing, and diagnostic use of the HIV-1 Gag gene
products expressed in Escherichia coli.
DT 9412
AU Shinagawa H; Saitoh A; Morimoto M; Nakata A; Research Institute for
Microbial Diseases, Osaka University,; Suita, Japan.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):248 (abstract no. PB0423). Unique
Identifier : AIDSLINE ICA10/94369891
AB OBJECTIVE: To purify and evaluate the various Gag proteins of HIV-1
produced in Escherichia coli as antigens for the diagnosis and
prognosis. METHODS: HIV-1 Gag proteins, p17, p24, p15, and p55, were
overproduced under the control of T7 promoter in E. coli and purified to
near homogeneity. Two-fold increasing amounts from 5 ng to 320 ng of
each of the Gag proteins were dotted on to the PVDF membrane and the
antigen dots were immunologically reacted with 1:100 dilution of the
sera of 40 HIV-1 carriers (AC 35, ARC 1, and AIDS 4). RESULTS: p55
reacted with all the 40 sera of HIV carriers. p17, p24, and p15 reacted
with 37, 35, and 34 of the 40 sera of HIV carriers, respectively. In
general, the sera of ARC and AIDS reacted more weakly with p17, p24, and
p55 than those of AC. The reaction of p55 was especially strong and that
of p15 was the weakest. In fact, p55 showed the strongest reaction in
all cases. DISCUSSION AND CONCLUSIONS: p55 is the most useful antigen
among the four Gag proteins for detection of Gag antibody in sera. The
methods to produce the HIV-1 Gag precursor and to process it to mature
products in vivo and in vitro were established.
DE Acquired Immunodeficiency Syndrome/DIAGNOSIS/IMMUNOLOGY AIDS
Serodiagnosis/*METHODS AIDS-Related Complex/DIAGNOSIS/IMMUNOLOGY
Escherichia coli/GENETICS Gene Products, gag/DIAGNOSTIC USE/*GENETICS
Human HIV Antigens/DIAGNOSTIC USE/*GENETICS HIV Core Protein
p24/DIAGNOSTIC USE/*GENETICS HIV Seropositivity/*DIAGNOSIS/IMMUNOLOGY
HIV-1/*GENETICS/IMMUNOLOGY Predictive Value of Tests Prognosis
Promoter Regions (Genetics) MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).